Identification of Pseudomonas viridiflava, causal agent of onion (Allium cepa L.) bulb rot

Objective: To phenotypically and molecularly characterize identify the causal agent of onion (Allium cepaL.) bulb rot in Morelos, Mexico.Methodology: Fluorescent bacteria from tissue with symptoms were isolated; LOPATtest was used to describe them subsequently they identified by partial 16S rRNA gene amplification.Pathogenicity vegetables plants evaluated injecting a suspension 108 CFU mL-1of pathogen.Results: Phenotypic characterization nucleotide sequencing showed 100% identity withPseudomonas viridiflava as rot. The pathogen caused infection broccoli (Brassica oleracea L.), spring fistulosum purple cepa cauliflower var. botrytis leek porrum carrot (Daucus carota well plant species such jalapeño pepper (Capsicum annuum bean (Phaseolus vulgaris tomato (Solanum lycopersicum L.).Implications: This information is important for agriculture Mexico. Pseudomonas bacterialpathogen high potential infect new hosts. first report P. causing Mexico.Conclusions: r